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junio 7, 2021

Survival of the bla<sub>NDM</sub>-harbouring Escherichia coli in tropical seawater and conjugative transfer of resistance markers

Anthropogenic contamination of coastal-marine water is responsible for introducing multidrug-resistant bacteria such as the pNDM-harbouring Escherichia coli into the seafood chain. This study was conducted to understand the survivability of a multidrug-resistant, the New Delhi Metallo-β-lactamase-producing E. coli (AS-EC121) in tropical seawater at room temperature (28-32 °C) compared to E. coli K12 strain. The experimental and control strains were inoculated at 6 log CFU/ml level into seawater….

A 3D printed Ga containing scaffold with both anti-infection and bone homeostasis-regulating properties for the treatment of infected bone defects

Large bone defects face a high risk of infection, which can also lead to bone homeostasis disorders. This seriously hinders the bone healing process; therefore, the help of a dual-functional scaffold that has both anti-infection and bone-homeostasis-regulating capacities is needed in the treatment of infected bone defects. In this study, a 3D printed dual-functional scaffold composed of poly-ε-caprolactone (PCL), mesoporous bioactive glasses (MBG), and gallium (Ga) was produced. In vitro…

<em>Bacillus subtilis</em> in PVA Microparticles for Treating Open Wounds

Open wound dressings should provide a moist environment, protect the wound from bacterial contamination, and shield it from further damage. These requirements, however, are hard to accomplish since such wounds are colonized by pathogenic bacteria, including resistant species such as methicillin-resistant Staphylococcus aureus (MRSA). A new approach for treating open wounds that is based on sticky and dissolvable polyvinyl alcohol (PVA) microparticles containing live Bacillus subtilis (B….

Zn(II)-tetracarboxy-phthalocyanine-Sensitized TiO<sub>2</sub> Thin Films as Antimicrobial Agents under Visible Irradiation: a Combined DFT and Experimental Study

In this article, we studied the antimicrobial activity of TiO(2) sensitized by the Zn(II)-tetracarboxy-phthalocyanine (TcPcZn) complex using TiO(2)-Degussa P25 as a semiconductor source. The TiO(2) thin films were deposited by the doctor blade method and were sensitized by the chemisorption process. The obtained compounds were characterized using Fourier transform infrared spectroscopy, UV-vis spectrophotometry, Raman spectroscopy, diffuse reflectance spectroscopy, and scanning electron…

The combined application of the anti-RAS1 and anti-RIM101 2′-OMethylRNA oligomers enhances Candida albicans filamentation control

Whereas antisense oligomers (ASOs) have been successfully utilized to control gene expression, they have been little exploited to control Candida albicans virulence’s determinants. Filamentation is an important virulence factor of C. albicans, and RAS1 and RIM101 genes are involved in its regulation. Thus, the main goal of this work was to project ASOs, based on 2′-OMethyl chemical modification, to target RAS1 and RIM101 mRNA and to validate its application either alone or in combination, to…

Antimicrobial consumption among hospitalized patients with COVID-19 in Pakistan

The discovery of different antimicrobial agents has revolutionized the treatment against a variety of infections for many decades, but the emergence of antimicrobial resistance require rigorous measures, even amid the coronavirus disease 2019 (COVID-19) pandemic. This retrospective study aimed to examine the consumption of antibiotics in patients with COVID-19 admitted into the five hospitals in the province of Punjab, Pakistan. We collected data on the consumption of antibiotics, classified…

Changing Results to Change Results: Nudging Antimicrobial Prescribing for Clostridium difficile

CONCLUSIONS: Microbiology reporting nudges raising the possibility of C difficile colonization were associated with altered prescribing, reinforcing a postanalytic strategy for invoking change. Decreases in antimicrobial prescribing occurred without increasing subsequent disease or other adverse outcomes, suggesting a safe strategy for decreasing unnecessary treatment of C difficile colonization.