A decline in the occurrence of Extended-spectrum β-Lactamase (ESBL)-producing Escherichia coli in retail chicken meat in the UK between 2013 and 2018.

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A decline in the occurrence of Extended-spectrum β-Lactamase (ESBL)-producing Escherichia coli in retail chicken meat in the UK between 2013 and 2018.

J Appl Microbiol. 2020 May 04;:

Authors: Randall LP, Horton RH, Chanter JI, Lemma F, Evans S

Abstract
AIMS: The aim of this study was to report on the occurrence antimicrobial resistant (AMR) E. coli from retail chicken meat samples in the UK, with particular focus on AmpC and ESBL production and carbapenem resistance.
METHODS AND RESULTS: Methods from EU protocols were used for selective isolation of AmpC-/ESBL-producing E. coli, carbapenem resistant E. coli and for performing MICs, but additional work not part of EU protocols included viable counts, detection by PCR of blaCTX-M , blaOXA, blaSHV and blaTEM genes in ESBL-phenotype E. coli and screening for mcr plasmid-mediated colistin resistance. From the 313/309 retail chicken meat samples tested in 2016/2018, carbapenem or mcr plasmid mediated colistin resistant E. coli were not detected. For 2016/2018 chicken samples, 141/42 (45.0/13.6), 90/23 (28.8%/7.4%), 48/16 (15.3%/5.2%) and 3/3 (1.0%/1.0%) were positive for ESBL- and/or AmpC-, ESBL- alone AmpC- alone and AmpC+ESBL-phenotype E. coli respectively. ESBL-producing E. coli were predominantly blaCTX-M-1 . All AmpC and or ESBL-phenotype E. coli were sensitive to colistin, ertapenem, imipenem, meropenem, temocillin and tigecycline, applying epidemiological cut-off values.
CONCLUSIONS: A previous study in 2013/14 showed that 65.4% of retail chicken meat samples tested in the UK were positive for ESBL-producing (mainly CTX-M) E. coli. Since then the proportion of samples positive in the UK has dropped significantly to 7.4% in 2018.
SIGNIFICANCE AND IMPACT OF STUDY: Significant reductions in antimicrobials used in the UK poultry meat sector between 2012 and 2016 may be linked to significant reductions in AmpC/ESBL-phenotype E. coli in retail chicken between 2013/14 and 2018.

PMID: 32364269 [PubMed - as supplied by publisher]