A hybrid plasmid formed by recombination of a virulence plasmid and a resistance plasmid in Klebsiella pneumoniae.

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A hybrid plasmid formed by recombination of a virulence plasmid and a resistance plasmid in Klebsiella pneumoniae.

J Glob Antimicrob Resist. 2020 Nov 16;:

Authors: Xie M, Dong N, Chen K, Yang X, Ye L, Chan EW, Zhang R, Chen S

Abstract
INTRODUCTION: The emergence of multidrug-resistant (MDR) and hypervirulent Klebsiella pneumoniae (hvKP) facilitates simultaneous dissemination of virulence and resistance in a single event which poses serious threat to public health.
METHOD: This study characterized the multidrug-resistant and moderately virulent ST11 K64 Klebsiella pneumoniae strain HB25-1 from a clinical case with microbiological and genomic approaches. Plasmids from strain HB25-1 were subjected to whole plasmid sequencing using both the Illumina NextSeq 500 sequencing platform and Nanopore MinION sequencer platforms. K. pneumoniae HB25-1 was subjected to conjugation experiment and Galleria mellonella infection model to evaluate the transmission and virulence potential.
RESULTS: We reported the emergence of an ST11, serotype K64 Klebsiella pneumoniae isolate, which was resistant to third-generation cephalosporin and exhibited a moderate level of virulence. WGS revealed that this strain harbored a plasmid, pHB25-1, which carried multidrug resistance genes (blaDHA-1, qnrB4, dfrA12, aadA2, sul1, aac(3)-lld, blaTEM-1, mph(E)) and virulence-encoding genes (the regulator of mucoid phenotype A gene rmpA2 and the aerobactin gene cluster iutAiucABCD). Genomic analysis indicated that pHB25-1 was formed through the co-integration of structural regions located in two different plasmids, enabling it to encode both resistance and virulent phenotypes.
CONCLUSION: Findings in this study provide evidence of active plasmid evolution in K. pneumoniae and suggest that surveillance of multidrug-resistant and hypervirulent K. pneumoniae is urgently needed.

PMID: 33212284 [PubMed - as supplied by publisher]