Application of High-Resolution Melting PCR to Detect the Genomic Fungal ITS 2 Region.

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Application of High-Resolution Melting PCR to Detect the Genomic Fungal ITS 2 Region.

Lab Med. 2020 Jan 02;51(1):66-73

Authors: Nawar NN, Behiry IK, Yousef RHA, Emara MA

Abstract
BACKGROUND: Invasive fungal infections (IFIs) are a main cause of morbidity and mortality. High-resolution melting polymerase chain reaction (HRM PCR) is promising for the identification of fungal species via the detection of internal transcribed spacer 2 (ITS2).
OBJECTIVES: To assess the sensitivity and specificity of HRM PCR in diagnosing IFIs, compared with blood culture.
METHODS: Our study included 100 patients who were suspected of having IFIs; we analyzed their specimens via blood culture and HRM PCR.
RESULTS: Blood culture results were positive in 57 cases and negative in 43 cases. HRM PCR results were positive in 14 cases and negative in 86 cases. The 14 cases with positive results included 4 with Candida tropicalis, 4 with Candida glabrata, and 6 with Candida krusei. HRM PCR sensitivity was 24.6%, specificity was 100%, positive predictive value (PPV) was 100%, and negative predictive value (NPV) was 50%.
CONCLUSIONS: HRM PCR is specific but not sensitive. Blood culture is more sensitive and cannot be replaced by HRM PCR.

PMID: 31198973 [PubMed - indexed for MEDLINE]