Aspirin or verapamil increased the sensitivity of Candida albicans to caspofungin under planktonic and biofilm conditions.

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Aspirin or verapamil increased the sensitivity of Candida albicans to caspofungin under planktonic and biofilm conditions.

J Glob Antimicrob Resist. 2020 Nov 23;:

Authors: Feng W, Yang J, Ma Y, Xi Z, Ren Q, Wang S, Ning H

Abstract
PURPOSE: The study aimed to investigate the effects of caspofungin (CAS) combined with aspirin (ASP) or verapamil (VPL) on the sensitivity of Candida albicans under planktonic and biofilm conditions.
METHODS: A total of 39 clinical strains were utilized to construct the biofilms of C. albicans. The drug sensitivity of ASP or VPL combined with CAS on C. albicans was analyzed by M27-A4 broth microdilution method. The MIC50 values were obtained and fractional inhibitory concentration index (FICI) was calculated. Afterwards, C. albicans ZY22 was selected for time-growth curve analysis, and ZY15 and ZY 22 were used for time-kill curve analysis.
RESULTS: Under planktonic condition, MIC50 of CAS was 0.0313-8 μg/mL after CAS alone treatment, while MIC50 of CAS was decreased to 0.0313-4 μg/mL after CAS combined with ASP or VPL. Under biofilm condition, MIC50 of CAS was 0.125-16 μg/mL when CAS alone treated, whereas MIC50 of CAS after CAS combined with ASP or VPL treatment was changed to 0.0625-16 μg/mL or 0.0625-8 μg/mL. FICI results showed that synergistic interactions between CAS and ASP under planktonic and biofilm conditions were severally observed in 17 and 16 strains. However, synergistic interactions between CAS and VPL under planktonic and biofilm conditions were respectively shown in 19 and 23 strains. Additionally, 8000 μg/mL ASP or 8 μg/mL VPL combined with CAS had better inhibitory effects on C. albicans.
CONCLUSION: ASP and VPL may be the sensitizer for CAS, and the antifungal effects of CAS may be sensitized by 8000 μg/mL ASP or 8 μg/mL VPL against C. albicans under planktonic and biofilm conditions.

PMID: 33242673 [PubMed - as supplied by publisher]