Candida parapsilosis complex from animals and its antifungal susceptibility and virulence attributes.
J Med Microbiol. 2014 Sep 4;
Authors: Brilhante RS, Rodrigues TD, Castelo-Branco DS, Teixeira CE, Macedo RB, Bandeira SP, Alencar LP, Monteiro AJ, Cordeiro RD, Bandeira TD, Moreira JL, Sidrim JJ, Rocha MF
This study aimed at identifying strains of the C. parapsilosis complex isolated from animals, as well as to assess their in vitro antifungal susceptibility profile and in vitro production of virulence attributes. We used 28 isolates of C. parapsilosis (sensu lato) recovered from clinically healthy animals. The strains were phenotypically characterized, followed by molecular identification of the species through PCR-Restriction Enzyme Analysis. Then, the susceptibility of the strains to amphotericin B, itraconazole, voriconazole, fluconazole and caspofungin was assessed through broth microdilution, according to CLSI (M27-A3). Additionally, the ability of the strains to produce biofilm, phospholipases and proteases was analyzed. Molecular analysis showed thirteen C. parapsilosis (sensu stricto), ten C. orthopsilosis and five C. metapsilosis strains. In vitro resistance to fluconazole was observed in three strains of C. parapsilosis (sensu stricto) and two C. metapsilosis. All tested strains were able to form biofilms, 23/28 isolates presented protease production, while none were able to produce phospholipases. Our study shows that C. parapsilosis sensu stricto and C. orthopsilosis are the most common species of the C. parapsilosis species complex and that these cryptic species present no significant phenotypical differences.
PMID: 25190736 [PubMed - as supplied by publisher]