Development and Optimization of TPGS based Stealth Liposome of Doxorubicin Using Box-Behnken Design: Characterization, Hemocompatibility and Cytotoxicity Evaluation in Breast Cancer Cells

J Liposome Res. 2021 Mar 16:1-46. doi: 10.1080/08982104.2021.1903034. Online ahead of print.

ABSTRACT

The present work reports the development of doxorubicin (DOX) encapsulated α-Tocopherol polyethylene glycol 1000 succinate (TPGS) coated liposomal system (DOX-LIPO-TPGS) by quality by design (QbD) approach and evaluated for its anticancer and hemocompatibility potential. The screening and optimization of formulation variables were performed by the systematic design of experiments (DoE), using Taguchi and Box-Behnken Design (BBD) for their desired quality attributes. The QbD optimized DOX-LIPO (DOX encapsulated uncoated liposome) and DOX-LIPO-TPGS formulation showed nano-metric vesicle size (98.2 ± 3.1 &117.6 ± 3.5 nm) with favorable development parameters, i.e. PDI (0.262 ± 0.008 & 0.123 ± 0.005); ZP (-38.7 ± 0.5 &-36.4 ± 0.7 mV) and % EE (66.8 ± 3.3 & 73.5 ± 3.5%) respectively. The release kinetics parameters suggested, sustained release behavior of developed liposomal formulations (83.6 ± 2.8 & 69.8 ± 2.2% releases in 72 h respectively). Cytotoxicity (MTT assay) on the MCF-7 breast cancer cell line and Hemolysis assay on RBCs stipulates comparatively higher anticancer potential and better hemocompatibility of DOX-LIPO-TPGS with respect to DOX-LIPO and the plain DOX solution. The study concluded that the QbD based three levels by three factors BBD optimization could be utilized for obtaining liposomal formulations with desired quality attributes. TPGS could be set out as a vital additive to improve the various quality parameters including stealthing character, stability, kinetic release, cytotoxicity, and hemocompatibility of liposomal formulations. This may serve as a focal paradigm for using TPGS coated liposomes as anticancer drug delivery vehicle in normal and MDR carcinoma.

PMID:33724151 | DOI:10.1080/08982104.2021.1903034