Diversity of antimicrobial resistance genes in Bacteroides and Parabacteroides strains isolated in Germany.
J Glob Antimicrob Resist. 2021 Jan 25;:
Authors: Rong SMM, Rodloff AC, Stingu CS
OBJECTIVES: The Bacteroides species are normally constituents of the human intestinal microflora, but they are also able to cause severe diseases. The aim of this study was to determine the diversity of resistance genes found in phenotypical resistant Bacteroides and Parabacteroides strains.
METHODS: A total of 71 phenotypical resistant Bacteroides spp. from human clinical specimens were screened for the following resistance genes cfiA, tetQ, tetM, tet36, cepA, cfxA, nim, ermG, ermF, bexA, blaVIM, blaNDM, blaKPC, blaOxa-48 and blaGES. The occurrence of these genes was compared with the phenotypic resistance to ampicillin-sulbactam, cefoxitin, ceftolozane-tazobactam, piperacillin-tazobactam, imipenem, meropenem, meropenem-vaborbactam, clindamycin, moxifloxacin, tigecycline, eravacycline and metronidazole.
RESULTS: TetQ was the most frequently detected gene, followed by cfiA, ermF, cfxA, ermG, cepA, nim and bexA. None of the strains were positive for tetM, tet36, blaVIM, blaNDM, blaKPC, blaOxa-48 and blaGES. Resistance to the tested ß-lactam antibiotics was mainly linked with the presence of the cfiA gene. Clindamycin resistance correlated with the presence of the genes ermG and ermF. The bexA gene was found in six strains, but only two of them were resistant to moxifloxacin. Tigecycline and eravacycline showed good activities despite the frequent occurrence of tetQ. The nim gene was detected in six isolates, five of them were resistant to metronidazole.
CONCLUSIONS: The findings of our study support the general belief that antimicrobial resistance within Bacteroides should be taken into consideration. This underlines the necessity of reliable routine susceptibility test methods for anaerobic bacteria and the implementation of antimicrobial surveillance programs worldwide.
PMID: 33508481 [PubMed - as supplied by publisher]