Draft genome sequence of a multidrug-resistant β-lactamase-harboringBacillus cereusS66, isolated from China.
J Glob Antimicrob Resist. 2019 Mar 04;:
Authors: Zhe S, Qianru Z, Liying Z, Zhidong Z, Ling J, He H
OBJECTIVES: Bacillus cereus is associated with a variety of diseases in the clinic and may cause human diseases such as hematological malignancies, tetanus, gas gangrene, food poisoning and pseudomembranous colitis. Our team found and isolated a strain called B. cereus S66 from radiation-contaminated soil in Xinjiang Uygur Autonomous Region. Here, we systematically studied and analyzed its drug resistance and toxicity.
METHODS: Genomic DNA ofB. cereus S66 was sequenced using the Illumina HiSeq 2000. After the Generated reads were assembled using SOAP denovo, the GapCloser was used to fill the gap area. The draft genome was annotated, virulence genes and drug resistance genes were identified by comparison with multi-class databases.
RESULTS: The genome of 5,628,370 bp and 34.84% GC content contains various toxicity and drug resistance related genes. The number of toxicity genes which are identified is 496, and some of those toxicity genes are associated with gastrointestinal infection, eye infections and neutrophil activating protein NapA. The drug resistance related genes reach 3.23% of the total genes and confer resistance to incomycin, tetracycline, fosfomycin, aminoglycoside, linezolid, chloramphenicol, rifampin, macrolide, daunorubicin and mupirocin. Three different β-lactamases were also found in this isolate.
CONCLUSIONS: In this report, the genome sequences ofB. cereus S66 provide a valuable reference to further research on multidrug resistance characteristics and pathogenic system analysis of Bacillus sp. in medicine.
PMID: 30844497 [PubMed - as supplied by publisher]