Effect and mechanism of citral against methicillin-resistant Staphylococcus aureus in vivo.
J Sci Food Agric. 2019 Mar 19;:
Authors: Long N, Tang H, Sun F, Lin L, Dai M
BACKGROUND: Citral is an active component of many plant extracts, and it's a safe additive which was used in food and cosmetics. Previous study showed citral has a good antibacteria effect against MRSA in vitro, but its in vivo anti-infective activity has not been studied. Anti-MRSA activity and the preliminary mechanism of citral against MRSA were investigated in MRSA infected KM mice. The ED50 was calculated (Karber's method), the groups were selected for inflammatory and oxidative stress level tests, and the lung and liver tissues were counterstained with HE, observe the pathological changes. Cytokines and oxidative factors were evaluated by ELISA method (one-way ANOVA computed by SPSS 19.0.).
RESULTS: With the increase of the concentration of citral, the survival rate of MRSA-infected mice increased accordingly. The ED50 values of citral for intramuscular injection and intragastric administration were 0.09 and 0.26 g kg -1 , respectively. Citral significantly reduced cytokines (IL-1β, IL-6, TNF-α) and oxidative factors (MDA, ·OH) of MRSA-infected mice, whereas increased GSH and SOD levels. Citral can reduced the lung inflammatory infiltrates infected by MRSA.
CONCLUSIONS: Citral exerted a dose-dependent anti-MRSA effect and ameliorated MRSA-induced abnormal changes in inflammation and oxidative stress. This indicates that citral has the potential to be developed as a new anti-MRSA drug. This article is protected by copyright. All rights reserved.
PMID: 30891759 [PubMed - as supplied by publisher]