Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores, and norovirus surrogate.
J Appl Microbiol. 2019 May 11;:
Authors: Wallace RL, Ouellette M, Jean J
AIMS: The current study aimed to assess the potential of a new high dose ultraviolet (UV) disinfection device to inactivate methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile and a norovirus surrogate on handheld mobile devices, and to compare the efficacy of the UV-C device to hydrogen peroxide disinfection wipes.
METHODS AND RESULTS: Suspensions of MRSA, C. difficile spores and a surrogate for norovirus (MS2) were inoculated onto glass or plastic coupons, with or without organic contamination and were exposed to continuous UV-C light for 15 to 60 s (165 to 646 mJ/cm2 ) in a self-contained UV-C chamber or treated with hydrogen peroxide wipes. Increasing the UV-C dose from 310 mJ/cm2 to 650 mJ/cm2 did not result in greater levels of inactivation. UV-C light inactivated all three microorganisms, in the absence of organic contamination, by >2.9 log. Treatment of MRSA, C. difficile spores, or MS2, in the presence of organic contamination, with UV-C light (310-646 mJ/cm2 ) resulted in 2.3-3.7 log reductions. Treatment of MRSA with UV-C light provided levels of inactivation comparable to treatment with hydrogen peroxide wipes used following the manufacturer's instructions.
CONCLUSIONS: UV-C light and hydrogen peroxide wipes had strong antimicrobial activity against MRSA, C. difficile spores and a norovirus surrogate, in the presence or absence of organic contamination. Significance and Impact: Chemical disinfection wipes are widely used in healthcare facilities, but they are not recommended for use on handheld mobile devices which may harbor pathogenic microorganisms. The powerful bactericidal, sporicidal, and virucidal activity of this high dose UV-C light device, shows that this technology is a promising alternative to chemical disinfectants, particularly for control of MRSA. This article is protected by copyright. All rights reserved.
PMID: 31077510 [PubMed - as supplied by publisher]