Etest versus vitek 2 vancomycin minimum inhibitory concentration testing methods for methicillin-resistant staphylococcus aureus: an antimicrobial stewardship initiative to evaluate the degree of discordance among methods at a rural tertiary hospital.

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Etest versus vitek 2 vancomycin minimum inhibitory concentration testing methods for methicillin-resistant staphylococcus aureus: an antimicrobial stewardship initiative to evaluate the degree of discordance among methods at a rural tertiary hospital.

J Pharm Pract. 2013 Aug;26(4):415-9

Authors: Bloomgren BJ, Laible BR

Abstract
PURPOSE: To evaluate the discordance between Vitek 2 and Etest vancomycin minimum inhibitory concentration (MIC) testing methods in methicillin-resistant Staphylococcus aureus (MRSA) isolates.
METHODS: Inclusion criteria consisted of MRSA isolates with blood, respiratory, or wound origin of culture, inpatient or emergency department location at time of culture and isolates with a Vitek 2 MIC reported. Isolates were subjected to Etest and the resulting MICs were compared to the corresponding Vitek 2 MICs, and both the rate and degree of discordance were evaluated.
RESULTS: Seventy-five MRSA isolates met the inclusion criteria. Etest resulted in an MIC value greater than that of Vitek 2 in 64 isolates (85.3%). Furthermore, of the 35 isolates with an MIC of ≤ 0.5 mcg/mL via Vitek 2, Etest reported 27 isolates with an MIC >1 mcg/mL. Of the 39 isolates with a Vitek 2 MIC of 1 mcg/mL, Etest reported 30 isolates with an MIC >1 mcg/mL.
CONCLUSION: We have demonstrated that Etest commonly reports MICs greater than 1 mcg/mL, even among isolates with MICs as low as ≤ 0.5 mcg/mL, according to Vitek 2. The clinical significance of these findings is unknown and would be an area of further study.

PMID: 23907075 [PubMed - indexed for MEDLINE]