Evaluation of magnesium oxide and zinc oxide nanoparticles against multi-drug-resistance Mycobacterium tuberculosis

Indian J Tuberc. 2021 Apr;68(2):195-200. doi: 10.1016/j.ijtb.2020.07.032. Epub 2020 Aug 4.

ABSTRACT

OBJECTIVE: The current study has evaluated the MICs and MBCs of ZnONPs, MgONPs, and MgONPs-ZnONPs against H37Rv Mtb and MDR-Mtb.

METHODS: Mixture, magnesium oxide nanoparticles (NPs) and zinc oxide (MgONPs-ZnONPs) were prepared. The microplate alamar blue (MABA) assay and the proportion method were used to evaluate of anti-tubercular activity against MDR-MTB. MTT test was done to MgONPs-ZnONPs against Vero and HepG2 cell lines.

RESULTS: The MIC of MgONPs and ZnONPs were 0.195 and 0.468 μg mL-1 against 104 of H37Rv Mtb. As well, 0.166 μg mL-1 of MgONPs-ZnONPs was able to inhibit 10-4 H37Rv Mtb. The MIC of MgONPs against 104 concentrations of MDR-Mtb was 12.5 μg mL-1. The MIC of MgONPs/ZnONPs against 104 concentrations of MDR-Mtb reached to 0.664 μg mL-1. The MBC value of ZnONPs increased to 1.875 μg mL-1 against 10-4 concentrations of MDR-Mtb. Testing showed that the MBCs of MgONPs/ZnONPs reached to 1.328 μg mL-1 against 104 concentrations of MDR-Mtb. The IC50 against MDR-TB was 0.779 μg mL-1 for ZnONPs and 0.883 μg mL-1 for MgONPs-ZnONPs. The MgONPs-ZnONPs was not toxic to Vero cell lines however ZnONPs could inhibit the Vero and HepG2 cell lines.

CONCLUSION: We found that ZnONPs and mixture MgONPs-ZnONPs not only have higher bactericide behavior but might have also synergistic effects against MDR-TB.

PMID:33845951 | DOI:10.1016/j.ijtb.2020.07.032