Fabrication of Phg/Gelatin/Silk Fibroin Nanofiber Scaffold Effective against Multidrug Resistant <em>Pseudomonas aeruginosa</em>

Drug Dev Ind Pharm. 2021 Jul 19:1-28. doi: 10.1080/03639045.2021.1957915. Online ahead of print.

ABSTRACT

The alarming rise of multi-drug resistant (MDR) Pseudomonas aeruginosa has prompted the World Health Organization to consider it a serious threat for human health. Although Phg (Phg), an effective antibacterial treatment option, can maintain long-term infectivity via lyophilized storage, freeze drying can be expensive and time consuming. Thus, we propose electrospun gelatin/fibroin (G/F) nanofibrous formulation for dehydrating and storing phage against MDR P. aeruginosa. Significance: The formulation of phage within the nanofibrous structure of the electrospun G/F scaffold would result in antimicrobial activity against MDR P. aeruginosa leading to enhanced wound healing. Methods: Phg effective against MDR P. aeruginosa was isolated, characterized and loaded within G/F nanofibers by electrospinning. Morphology, crystallinity and thermal stability as well as the antimicrobial activity and the biocompatibility of the developed G/F/Phg nanofibers were determined. Results: Phg-loaded G/F nanofibers revealed an amorphous structure with good thermal stability at temperatures below 300 °C and exhibited effective antibacterial activity against MDR P. aeruginosa with ∼2 log reduction in bacterial count which increased to ∼4 log reduction in bacterial count after 16h as compared to both the G/F nanofibers and the negative control. Lack of cytotoxic effects on cultured fibroblasts supported the biocompatibility of G/F/Phg nanofibers. Conclusion: The developed G/F/phg are able to maintain the viability of phage and represent a promising antimicrobial dressing for wounds infected with MDR P. aeruginosa.

PMID:34278896 | DOI:10.1080/03639045.2021.1957915