Ertapenem

An insight into analysis and elimination of plasmids that encodes metallo-β-lactamases in Pseudomonas aeruginosa.

J Glob Antimicrob Resist. 2019 Sep 10;:

Authors: Paul D, Dhar Chanda D, Chakarvarty A, Bhattacharjee A

Abstract
OBJECTIVES: The aim of this study was to characterize the metallo-β-lactamase harboring plasmid, their alteration in copy number in respect to different antibiotic pressure as well as the efficiency of different curing agents in eliminating these resistant plasmids from nosocomial isolates of Pseudomonas aeruginosa.
METHODS: Plasmids were extracted from blaNDM-1 &blaVIM-2 harboring isolates under four different concentrations of imipenem, meropenem and ertapenem. Quantitative real time PCR was performed to analyze the alteration in the plasmid copy number under these different conditions. Plasmids carrying blaNDM-1 and blaVIM-2 were tried to eliminate using different physical and chemical curing agents and meropenem resistance was used as the selectable marker.
RESULTS: Conjugatively transferable metallo-beta-lactamase genes (blaNDM-1 &blaVIM-2) were found to be highly stable and in this study SDS was found to be the most effective agents that could able to eliminate these resistance plasmids. The copy number alteration of blaNDM-1 encoding plasmid was found to be similar with the blaVIM-2 with a single exception under cefotaxime pressure.
CONCLUSION: The spread of multidrug resistant plasmid has been noted as the key factor associated with the elevating carbapenem resistance and the successful curing of a resistance plasmid can reverse back the phenotype into a susceptible one. This study revealed that concentration gradient antibiotic pressure induces the copy number of the metallo-beta lactamase encoding plasmids and also a specific concentration of SDS can be successfully used as an eliminating agent for these resistance determinants.

PMID: 31518725 [PubMed - as supplied by publisher]