Hypermucoviscous Polymyxin Resistant Klebsiella pneumoniae from Kolkata, India: Genomic and Phenotypic Analysis.

Hypermucoviscous Polymyxin Resistant Klebsiella pneumoniae from Kolkata, India: Genomic and Phenotypic Analysis.

J Glob Antimicrob Resist. 2019 Feb 27;:

Authors: Bean DC, Agarwal A, Cherian BP, Wareham DW

Abstract
OBJECTIVES: Here we report the draft genome sequence of a colistin resistant hypermucoviscous Klebsiella pneumoniae isolated from a hospitalised patient with acute kidney injury in Kolkata, India.
METHODS: Whole genomic DNA was sequenced using an Illumina HiSeq platform. The generated reads were de novo assembled using SPAdes version 3.7.1. Annotation was performed by the NCBI Prokaryote Genome Annotation Pipeline version 4.6. Sequence type, capsular type, antimicrobial resistance and virulence-related genes were predicted from the sequence.
RESULTS: K. pneumoniae KP26 belonged to sequence type (ST) 147. The assembly comprised 63 contigs (>1,000bp) with a total length of 5,560,935bp, and a total of 5,399 coding sequences. The isolate was resistant to most β-lactams, aminoglycosides, quinolones, fosfomycin, trimethoprim, sulphonamides and polymyxins. No mcr genes were detected in the genome.
CONCLUSION: Isolate KP26 is a hypermucoviscous, multidrug-resistant strain of K. pneumoniae that may represent an emerging high risk clone associated with severe infections in India.

PMID: 30825699 [PubMed - as supplied by publisher]