Identification of a U/Zn/Cu responsive global regulatory two-component system in Caulobacter crescentus.

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Identification of a U/Zn/Cu responsive global regulatory two-component system in Caulobacter crescentus.

Mol Microbiol. 2016 Dec 30;:

Authors: Park DM, Overton KW, Liou MJ, Jiao Y

Abstract
Despite the well-known toxicity of uranium (U) to bacteria, little is known about how cells sense and respond to U. The recent finding of a U-specific stress response in Caulobacter crescentus has provided a foundation for studying the mechanisms of U- perception in bacteria. To gain insight into this process, we used a forward genetic screen to identify the regulatory components governing expression of the urcA promoter (PurcA ) that is strongly induced by U. This approach unearthed a previously uncharacterized two-component system, named UzcRS, which is responsible for U-dependent activation of PurcA . UzcRS is also highly responsive to zinc and copper, revealing a broader specificity than previously thought. Using ChIP-seq, we found that UzcR binds extensively throughout the genome in a metal-dependent manner and recognizes a non-canonical DNA binding site. Coupling the genome-wide occupancy data with RNA-seq analysis revealed that UzcR is a global regulator of transcription, predominately activating genes encoding proteins that are localized to the cell envelope; these include metallopeptidases, multidrug resistant efflux (MDR) pumps, TonB-dependent receptors and many proteins of unknown function. Collectively, our data suggest that UzcRS couples perception of U, Zn and Cu with a novel extracytoplasmic stress response. This article is protected by copyright. All rights reserved.

PMID: 28035693 [PubMed - as supplied by publisher]