Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein.
J Microbiol Methods. 2015 Jan;108:61-9
Authors: Kaba HE, Maier N, Schliebe-Ohler N, Mayer Y, Müller PP, van den Heuvel J, Schuchhardt J, Hanack K, Bilitewski U
We selected the immunogenic cell wall ß-(1,3)-glucosyltransferase Bgl2p from Candida albicans as a target protein for the production of antibodies. We identified a unique peptide sequence in the protein and generated monoclonal anti- C. albicans Bgl2p antibodies, which bound in particular to whole C. albicans cells.
PMID: 25451457 [PubMed - indexed for MEDLINE]