Immunological and histopathological characterisation of cutaneous candidiasis.
J Med Microbiol. 2015 Jun 1;
Authors: Campois TG, Zucoloto AZ, Araujo EJ, Svidizinski TI, Almeida RS, Quirino GF, Harano RM, Conchon-Costa I, Felipe I
Chronic mucocutaneous candidiasis constitutes a heterogeneous group of syndromes, characterised by non-invasive infection of the skin, nails, and mucosal membranes by the fungus, Candida spp. Though symptoms are heterogeneous, in all cases there is a reduction in protective cytokines, favouring the development of disease. The normal role of cytokines at skin lesions is not well understood. The presents tudy aimed to investigate the progression of disease, understand specific cellular and molecular components involved in immunity to Candida albicans,and to determine the balance between pro- and anti-inflammatory cytokines over the course of cutaneous infection in immunocompetent mice. Balb/c mice (five per group) were inoculated with 5 × 106 C. albicans pseudohyphae in the deep dermis of the paw from1to14 d. The contralateral paws were used for negative controls. Hematoxylin-Eosin staining of skin sections during C. albicans infection was performed to analyse structural modifications to the epidermis such as hyperplasia, and infiltration of neutrophils and fibroblasts in the dermis. The cytokine populations were determined by capture ELISA using popliteal lymph node tissue. Pro-inflammatory cytokines (IL-6, TNF-α, IL-12, IFN-γ, IL-17) were detected at significant levels during the initial phase of cutaneous infection and correlated with the rapid elimination of C. albicans. Anti-inflammatory cytokines (IL-13, IL-4, IL-10,and TGF-β)were detected on day four post-infection, and prevented exacerbation of inflammation and participated in healing of lesions. Thus, a balance between pro- and anti-inflammatory cytokines was fundamental for the resolution of infection. Importantly, these findings broaden the understanding of the immune mechanisms involved in chronic cutaneous candidiasis.
PMID: 26032158 [PubMed - as supplied by publisher]