Impact of Brief Exposure to Drugs with Antifungal Properties on the Susceptibility of Oral Candida dubliniensis Isolates to Lysozyme and Lactoferrin.

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Impact of Brief Exposure to Drugs with Antifungal Properties on the Susceptibility of Oral Candida dubliniensis Isolates to Lysozyme and Lactoferrin.

Med Princ Pract. 2018 Sep 02;:

Authors: Ellepola ANB, Dassanayake R, Khan ZU

Abstract
OBJECTIVE: Lysozyme and lactoferrin have anti-candidal activity. Candida dubliniensis is associated with oral candidiasis. Candida infections are managed with nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole and chlorhexidine. Candida undergo a brief exposure to therapeutic agents in the mouth. There is no data on the influence of limited exposure to antimycotics on the sensitivity of C. dubliniensis to lactoferrin and lysozymes. Hence, this study observed the changes in the sensitivity of C. dubliniensis to anti-candidal action of lactoferrin and lysozyme after transitory exposure to sub-lethal concentrations of antifungals.
MATERIALS AND METHODS: After determination of the minimum inhibitory concentration (MIC), twenty C. dubliniensis isolates were exposed to twice the concentration of MIC, of nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole and chlorhexidine for 1 hour. Drugs were removed by dilution and thereafter the susceptibility of these isolates to lysozyme and lactoferrin was determined by colony forming unit quantification assay.
RESULTS: Exposure of C. dubliniensis to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole and chlorhexidine resulted in an increase in susceptibility to lysozyme by 9.45%, 30.82%, 30.04%, 50.64%, 55.60% and 50.18%, respectively (p < 0.05 to p < 0.001). Exposure of C. dubliniensis to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole and chlorhexidine resulted in an increase of susceptibility to lactoferrin by 13.54 %, 16.43 %, 17.58 %, 19.60 %, 21.32 % and 18.73 %, respectively (p < 0.05 to p < 0.001).
CONCLUSION: Brief exposure to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole and chlorhexidine enhances the antifungal effect of lysozyme and lactoferrin on C. dubliniensis isolates in vitro.

PMID: 30173211 [PubMed - as supplied by publisher]