Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China.

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Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China.

J Med Microbiol. 2015 Jan 16;

Authors: Shi XY, Yang YP, Zhang Y, Li W, Wang JD, Huang WM, Fan YM

Abstract
 There is limited information regarding molecular epidemiology and antifungal susceptibilities of Candida isolates using Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using Neo-Sensitabs tablet diffusion method. Candida species were isolated from 186 cases (61.79%). The most common pathogen was C. albicans (91.4%), followed by C. glabrata (4.3%), C. tropicalis (3.2%), and C. parapsilosis (1.1%). The susceptible rates of C. albicans were higher for caspofungin, voriconazole and fluconazole than those for itraconazole, miconazole ketoconazole and terbinafine (P<0.01). The resistant rates of C. albicans were 4.7%, 6.5%, 7.1%, 7.6%, 12.3%, 27.7%, and 74.7% for caspofungin, miconazole, itraconazole, voriconazole, fluconazole, ketoconazole, and terbinafine, respectively. All drugs tested but fluconazole exhibited no differences in the resistance between C. albicans and non-albicans Candida isolates. The results demonstrate that C. albicans is the most common isolate from Chinese patients with VVC by DNA sequencing. Caspofungin, voriconazole and fluconazole may be preferable to other azoles and terbinafine in the treatment of VVC.

PMID: 25596116 [PubMed - as supplied by publisher]