Nepodin inhibits biofilm formation by Candida albicans and polymicrobial microorganisms via hyphal growth suppression.

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Nepodin inhibits biofilm formation by Candida albicans and polymicrobial microorganisms via hyphal growth suppression.

ACS Infect Dis. 2019 May 05;:

Authors: Lee JH, Kim YG, Khadke SK, Yamano A, Watanabe A, Lee J

Abstract
Candida albicans is an opportunistic pathogenic yeast and is responsible for candidiasis. It readily colonizes host tissues and implant devices, and forms biofilms, which play an important role in pathogenesis and drug resistance. In this study, the antibiofilm, antihyphal, and antivirulence activities of nepodin isolated from Rumex japonicus roots, was investigated against a fluconazole-resistant C. albicans strain and polymicrobial microorganisms biofilm formation. Nepodin effectively inhibited C. albicans biofilm formation without affecting its planktonic cell growth. Also, Rumex root extract and nepodin both inhibited the hyphal growth and cell aggregation of C. albicans. Interestingly, nepodin also showed antibiofilm activities against Candida glabrata, Candida parapsilosis, Staphylococcus aureus and Acinetobacter baumannii strains and dual biofilms of C. albicans and S. aureus or A. baumannii, but not against Pseudomonas aeruginosa. Transcriptomic analysis performed by RNA-Seq and qRT-PCR showed nepodin repressed the expressions of several hypha/biofilm related genes ( ECE1, HGT10, HWP1, and UME6) and increased the expressions of several transport genes ( CDR4, CDR11, and TPO2), which supported phenotypic changes. Moreover, nepodin reduced C. albicans virulence in a nematode infection model and exhibited minimal cytotoxicity against the nematode and an animal cell line. These results demonstrate nepodin and Rumex root extract might be useful for controlling C. albicans infections and multispecies biofilms.

PMID: 31055910 [PubMed - as supplied by publisher]