Phospholipase and aspartyl proteinase activities of Candida species causing vulvovaginal candidiasis in patients with type 2 diabetes mellitus.

Phospholipase and aspartyl proteinase activities of Candida species causing vulvovaginal candidiasis in patients with type 2 diabetes mellitus.

J Microbiol Biotechnol. 2015 Jun 2;

Authors: Bassyouni RH, Wegdan AA, Abdelmoneim A, Said W, AboElnaga F

Abstract
Few researches had investigated the secretion of phospholipase and aspartyl proteinase from Candida spp. causing infection in females with type 2 diabetes mellitus. This research aimed to investigate the prevalence of vulvovaginal candidiasis (VVC) in diabetic versus non-diabetic women and compare the ability of identified Candida isolates to secrete phospholipases and aspartyl proteinases with characterization of their genetic profile. The study included 80 females with type 2 diabetes mellitus and 100 non diabetic females within child bearing period. Candida strains were isolated and identified by conventional microbiological methods and by API ® Candida. The isolates were screened for their extracellular phospholipase and proteinase activities by culturing them on egg yolk and bovine serum albumin media respectively. Detection of aspartyl proteinase genes (SAP1 to SAP8) and phospholipase genes (PLB1, PLB2) were performed by multiplex polymerase chain reaction. Our results indicated that vaginal candidiasis was significantly higher among diabetic group versus non-diabetic group (50% versus 20% respectively) (P=0.004). C. albicans was the most prevalent species followed by C. glabrata in both groups. No significant association between diabetes mellitus and phospholipase activities was detected (P=0.262) while high significant proteinase activities exhibited by Candida isolated from diabetic females were found (82.5%) (P=0.000). Non-significant association between any of tested proteinase or phospholipase genes and diabetes mellitus were detected (P>0.05). In conclusion it is noticed that incidence of C. glabrata causing VVC is increased. The higher prevalence of vaginal candidiasis among diabetics could be related to increased aspartyl proteinases production in this group of patients.

PMID: 26032358 [PubMed - as supplied by publisher]