Prevalence and Multidrug Resistance Pattern of Salmonella Carrying Extended-Spectrum β-Lactamase in Frozen Chicken Meat in Bangladesh.

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Prevalence and Multidrug Resistance Pattern of Salmonella Carrying Extended-Spectrum β-Lactamase in Frozen Chicken Meat in Bangladesh.

J Food Prot. 2020 Jul 14;:

Authors: Parvin MS, Hasan MM, Ali MY, Chowdhury EH, Rahman MT, Islam MT

Abstract
Salmonella is an important foodborne pathogen that causes public health problem globally, and chicken meat is considered as an important reservoir of Salmonella dissemination to humans. Furthermore, the increase of antimicrobial resistance (AMR) in Salmonella has intensified the problem. This study aimed at estimating the burden of Salmonella carrying extended-spectrum β-lactamase (ESBL) and their AMR pattern in 113 domestic frozen chicken meat samples purchased from supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of β-lactamase-, and plasmid-mediated quinolone resistance-encoding genes. All samples were analyzed for the presence of Salmonella using selective media and PCR assay. Antimicrobial susceptibility test was done by disk diffusion test, and ESBL screening was performed by double disk synergy tests. Resistance genes were detected using multiplex PCR. The results showed that 65.5% samples were positive for Salmonella spp., of which, 58.1% isolates were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to both 3-5 and 6-8 antimicrobial classes was observed in 40.5% isolates, while 17.6% were resistant to 9-11 classes, and 1.4% isolates to 12-15 classes. The highest rates of resistance were observed against oxytetracycline (100%), followed by trimethoprim-sulphamethoxazole (89.2%), tetracycline (86.5%), nalidixic acid (83.8%), amoxicillin (74.3%), and pefloxacin (70.3%). Notably, 48.6% of isolates demonstrated resistance to imipenem. One (1.4%) isolate was possible extensively drug resistant. All the isolates were positive for the blaTEM gene while 2.7% were positive for blaCTX-M-1 and 20.3% for blaNDM gene. The prevalence of qnrA and qnrS genes was 4.1% and 6.8%, respectively. This study shows that ESBL-producing Salmonella spp. are widespread in frozen chicken meat in Bangladesh, which puts greater responsibility on food processors, and policy makers to ensure food safety.

PMID: 32663273 [PubMed - as supplied by publisher]