Recommendations to address the difficulties encountered when determining linezolid resistance from whole genome sequencing data.

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Recommendations to address the difficulties encountered when determining linezolid resistance from whole genome sequencing data.

Antimicrob Agents Chemother. 2018 May 29;:

Authors: Beukers AG, Hasman H, Hegstad K, van Hal SJ

Abstract
Mutations associated with linezolid resistance within the V domain of 23S rRNA are annotated using an Escherichia coli numbering system. The 23S rRNA gene varies in length, nucleotide sequence and copy number between bacterial species. Consequently, this numbering system is not intuitive and can lead to confusion when locating mutation sites using whole genome sequencing data. Using the mutation G2576T as an example, we demonstrate the difficulties associated with using the E. coli numbering system.

PMID: 29844046 [PubMed - as supplied by publisher]