Research Techniques Made Simple: Mouse Bacterial Skin Infection Models for Immunity Research.
J Invest Dermatol. 2020 May 11;:
Authors: Youn C, Archer NK, Miller LS
Bacterial skin infections are a major societal health burden and are increasingly difficult to treat due to the emergence of antibiotic resistant strains such as community-acquired methicillin-resistant Staphylococcus aureus. Understanding the immunological mechanisms that provide durable protection against skin infections has the potential to guide the development of immunotherapies and vaccines to engage the host immune response to combat these antibiotic resistant strains. To this end, mouse skin infection models allow researchers to examine host immunity by investigating the timing, inoculum, route of infection and the causative bacterial species in different wildtype mouse backgrounds as well as in knockout, transgenic and other types of genetically engineered mouse strains. To recapitulate the various types of human skin infections, many different mouse models have been developed. For example, four models frequently used in dermatological research are based on route of infection, including: (i) subcutaneous infection models, (ii) intradermal infection models, (iii) wound infection models, and (iv) epicutaneous infection models. In this article, we will describe these skin infection models in detail along with their advantages and limitations. Additionally, we will discuss how humanized mouse models such as the human skin xenograft on immunocompromised mice might be used in bacterial skin infection research.
PMID: 32407714 [PubMed - as supplied by publisher]