Resistance and cross-resistance in Staphyloccocus sp. after prolonged exposure to different antiseptics.
J Glob Antimicrob Resist. 2019 Nov 04;:
Authors: Nicolae Dopcea G, Dopcea I, Nanu A, Diguta C, Matei F
OBJECTIVES: This study aimed to evaluate the potential of staphylococci to develop resistance and cross-resistance to antibiotics after exposure to antiseptic.
METHODS: Antibiotic susceptibility profile, MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were measured for Staphylococcus epidermidis and Staphylococcus aureus clinical or of collection strains, methycilin resistant or not, before and after prolonged exposure to low-level concentration of two antiseptics (chlorhexidine digluconate, octenidine dihydrochloride).
RESULTS: Resistance to antibiotics was observed in both tested antiseptics following exposure. Chlorhexidine exposure, led to increased MICs in five of the tested strains from both S. epidermidis and S. aureus and also led to clinical decreased susceptibility to gentamicin in Staphylococcus aureus (ATCC 43300) and to penicillin and tetracycline in Staphylococcus aureus (ATCC 25923). Octenidine exposure, led to increased MICs in Staphylococcus epidermidis (ATCC 12228) and also led to a significant decreased susceptibility to ceftazidime Staphylococcus aureus (SAL). One strain (Staphylococcus epidermidis ATCC 12228) has registered a 4 folds increased of the MIC against CHG and few strains have had 2 folds' increase against CHG and only one strain (Staphylococcus epidermidis ATCC 12228 again) against OCT.
CONCLUSIONS: The obtained results are supporting the urgent need to apply the same administration rules accepted currently for the antibiotics, taking provisions to be preserved the benefits of both antiseptics and antibiotics.
PMID: 31698107 [PubMed - as supplied by publisher]