Resistome and pathogenomics of multidrug resistant(MDR) Pseudomonas aeruginosa VRFPA03, VRFPA05 recovered from alkaline chemical keratitis and post-operative endophthalmitis patient.

Resistome and pathogenomics of multidrug resistant(MDR) Pseudomonas aeruginosa VRFPA03, VRFPA05 recovered from alkaline chemical keratitis and post-operative endophthalmitis patient.

Gene. 2015 Dec 9;

Authors: Murugan N, Malathi J, Umashankar V, Madhavan HN

Abstract
Eye infections due to Pseudomonas aeruginosa is an important cause of ocular morbidity. we presents the whole genomic comparative analysis of two P. aeruginosa VRFPA03 and VRFPA05 isolated from alkaline chemical injury mediated keratitis and Post-cataract surgery endophthalmitis patients respectively. The blaDIM-1 gene in VRFPA03 and the blaGes-9 gene in VRFPA05 were identified and reported for the first time from an ocular isolate. The current study revealed novel integrons In1107 and In1108, comprised of multi-drug resistant genes. Ocular virulence factors mainly mediated by exoenzymes T, Y, and U and exotoxin A, elastase B, and phenazine-specific methyltransferase. Genomic analysis uncovered multiple known and unknown factors involved in P. aeruginosa mediated ocular infection, which may lead to drug discovery and diagnostic markers to improve human vision care.

PMID: 26692145 [PubMed - as supplied by publisher]