SCCmec IX in methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci from pigs and workers at pig farms in Khon Kaen, Thailand.

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SCCmec IX in methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci from pigs and workers at pig farms in Khon Kaen, Thailand.

J Med Microbiol. 2015 Jun 30;

Authors: Sinlapasorn S, Lulitanond A, Angkititrakul S, Chanawong A, Wilailuckana C, Tavichakorntrakool R, Chindawong K, Seelaget C, Krasaesom M, Chartchai S, Wonglakorn L, Sribenjalux P

Abstract
INTRODUCTION: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA), Clonal Complex (CC) 398, has been reported in Europe, whereas CC9 MRSA has mostly been found in Asia. We therefore aimed to detect MRSA on pig farms in northeastern Thailand.
METHODS: A total of 257 nasal swabs (159 samples from pigs and 98 from pig farm workers) were collected from 3 pig farms in northeastern Thailand from 2010-2011. MRSA isolates were confirmed for femA and mecA genes by PCR. The minimum inhibitory concentrations of eight antimicrobials, namely vancomycin, cefazolin, ofloxacin, tetracycline, erythromycin, oxacillin, cefoxitin and gentamicin, were tested by agar dilution method. The virulence genes for Panton-Valentine leukocidin toxin (lukSF-PV), toxic shock syndrome toxin-1 (tst) and α-hemolysin (hla) were detected by PCR. Strain typing was performed by Staphylococcal cassette chromosome (SCC) mec, agr, spa, and multilocus sequence typing.
RESULTS: Four MRSAs were isolated: 3 from workers and one from a pig. All the MRSAs were resistant to oxacillin, gentamicin, erythromycin, tetracycline, and cefazolin, and they all carried hla only. Two MRSAs from humans carried SCCmecII-ST764-agrII, whereas the two remaining MRSAs (one each from a human and a pig) contained SCCmec IX-ST9-agrII. Interestingly, methicillin-resistant coagulase-negative Staphylococcus isolates carrying SCCmec IX were also obtained from 5 workers and 3 pigs.
CONCLUSIONS: This study suggests that the SCCmec IX element is distributed among the Staphylococcus found in pigs and pig farm workers, and pigs may be a reservoir for MRSA in the community.

PMID: 26296763 [PubMed - as supplied by publisher]