Tracing the dissemination of the international clones of multi-drug-resistant Acinetobacter baumannii among cancer patients in Egypt using the PCR-based open reading frame typing (POT) method.
J Glob Antimicrob Resist. 2019 Aug 02;:
Authors: El Far MY, El-Mahallawy HA, Attia AS
OBJECTIVES: The aim of this study was to perform an epidemiological surveillance of multi-drug-resistant (MDR) A. baumannii genetic lineages among cancer patients in Egypt using the PCR-based open reading frame typing (POT) method.
METHODS: A total of 160 MDR A. baumannii isolates were collected between January 2015 and December 2017 at a tertiary centre. Vitek2® system was used for preliminary species identification and susceptibility testing. The PCR-based open reading frame typing (POT) method was applied for confirmation of species identification and molecular epidemiological typing of the isolates.
RESULTS: MDR A. baumannii isolates were classified into 15 POT types including POT 122 (n = 69), POT 69 (n = 22), and other miscellaneous POT types (MOPTs) including POT numbers (0, 8, 10, 12, 14, 40, 44, 52, 56, 93, 104, 106, and 108) (n = 69). POT 122 isolates infected or colonized 61% of the patients hospitalized in surgical wards, 54% of the patients diagnosed with solid tumours and 51% were adults. On the other hand, MPOTs isolates infected or colonized 51% of the patients hospitalized in paediatric wards, 49% of the patients diagnosed with haematological malignancies and 51% were paediatric patients (p = 0.007, 0.001, and 0.004, respectively). MPOTs isolates were recovered from 46% of the collected blood specimens, while POT 122 isolates were recovered from 61% of the collected respiratory specimens (p = 0.05).
CONCLUSION: The current study demonstrated that the low-cost easy POT method is convenient for rapid delineation of A. baumannii clonal diversity in a tertiary care hospital in Egypt.
PMID: 31382074 [PubMed - as supplied by publisher]