Vaginal Epithelial Cells discriminate between yeast and hyphae of C. albicans in women who are colonized with or have vaginal candidiasis.
J Infect Dis. 2019 Jul 11;:
Authors: Roselletti E, Perito S, Sabbatini S, Monari C, Vecchiarelli A
BACKGROUND: Vaginal candidiasis is common disease affecting women; however, how C. albicans shift from commensalism towards a pathogenic status remains poorly understood. The present study investigated the vaginal epithelial cell (EC) response dynamics under various conditions.
METHODS: Healthy women, asymptomatic C. albicans carriers, and symptomatic patients with vaginal candidiasis were enrolled in this study. ECs in the vaginal swabs were analyzed with cytofluorimetric analysis for pattern recognition receptors and intracellular signals, with LDH performed for cell damage and an ELISA for cytokine expression.
RESULTS: The level of TLR4, TLR2, and EphA2 expression was significantly higher in ECs from asymptomatic and symptomatic subjects compared to healthy donors. Activation of the transcription factors NF-B and c-Fos-p-38 was observed in ECs from symptomatic and asymptomatic pseudo-hyphae/hyphae carriers, but not from the asymptomatic yeast carriers. EC damage was only observed in symptomatic patients.
CONCLUSION: The presence of pseudo hyphae/hyphae is required to determine vaginal candidiasis; however, it may be not sufficient to induce the pathological process associated with neutrophil recruitment and EC damage. This study sheds light into the ambiguous role of the hyphal form during vaginal human-commensalism.
PMID: 31300818 [PubMed - as supplied by publisher]