Whole genome sequencing of Escherichia coli isolated from contaminated meat samples collected from the Northern Region of Ghana revealed the presence of multidrug resistant genes.

Related Articles

Whole genome sequencing of Escherichia coli isolated from contaminated meat samples collected from the Northern Region of Ghana revealed the presence of multidrug resistant genes.

J Glob Antimicrob Resist. 2019 Mar 26;:

Authors: Adzitey F, Assoah-Peprah P, Teye GA

Abstract
OBJECTIVES: This study reports the draft genomes of 14 Escherichia coli (E. coli) isolated from contaminated meat samples collected from the Northern Region of Ghana, in order to determine the presence of antibiotic resistant genes and their genetic relatedness.
METHODS: The 14 E. coli were of beef (n = 3), mutton (n = 2), chevon (n = 3), local fowl (n = 3) and guinea fowl (n = 3) origin. Whole genome sequencing (WGS) was performed using Illumina MiSeq sequencer. A double-disk synergy test (DDST) was also used to confirm the production of extended-spectrum-beta-lactamase (ESBL).
RESULTS: WGS confirmed the identity of all the E. coli isolates. All the E. coli strains contained at least one resistant gene and 57.1% of them were multidrug resistant (MDR). The mdf(A) gene was most common resistant gene observed. DDST confirmed the presence of ESBL gene (blaCTX-M-15) in a MDR E. coli of guinea fowl origin. The sequence type (ST) genetically varied among the E. coli strains, except three strains that had the same STs of 155. Similarly, the serotypes of the meat sample E. coli strains were genetically diverse. Eleven different plasmid sequences were detected in 10 of the E. coli strains.
CONCLUSION: This work showed that E. coli from contaminated meat sources in Ghana possessed multidrug resistant genes and were genetically diverse. To the best of our knowledge, this is the first work on whole genome sequencing of E. coli isolated from various meat samples in our study area; and sequence data adds to data base for epidemiological studies.

PMID: 30926467 [PubMed - as supplied by publisher]